Journal: eBioMedicine
Article Title: Reprogramming offspring liver health: maternal indole supplementation as a preventive strategy against MASLD
doi: 10.1016/j.ebiom.2025.106098
Figure Lengend Snippet: Hepatic AHR signalling is restored in adult WD-challenged offspring with perinatal exposure to Ind and I3A, concomitant with reduced fibrogenesis. ( a ) Protein levels by Simple Western analysis for AHR and CYP1A1. Representative images and densitometry quantification are shown. Vinculin was the loading control for AHR and GAPDH for CYP1A1. Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test. Hepatic (whole liver) gene expression analysis for de novo lipogenesis genes ( Srebp1c , Fasn , and Acc1 ) and lipid transport ( Cd36 ) ( b ), genes associated with hepatic stellate cell activation and liver fibrosis ( c ), inflammatory genes ( d ), and genes associated with monocyte infiltration ( e ) by qPCR, normalised to Tbp . Data are mean ± SEM, n = 6–9/group (3–5 litters/group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 vs. WD by one-way ANOVA with Dunnett's test, except Acta2 , Timp1 , and Ccr2 by Kruskal–Wallis with Dunn's test. ( f ) Gene expression of genes in isolated hepatocytes by qPCR, normalised to Rpl13a . Data are mean ± SEM, n = 9/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. Gene expression of genes associated with AHR ( Cyp1a2 ) and oxidative defence ( g ) and inflammatory genes ( h ) in MerTK + liver macrophages by qPCR, normalised to Actb . Data are mean ± SEM, n = 6–12/group (3–6 litters/group). ∗p < 0.05 vs. WD by one-way ANOVA with Dunnett's test. ( i ) Gene expression of genes involved in fibrosis in hepatic stellate cells (HSCs) by qPCR, normalised to Rn18s . Data are mean ± SEM, n = 3–7/group (3 litters/group). ∗p < 0.05 and ∗∗p < 0.01 vs. WD by one-way ANOVA with Dunnett's test.
Article Snippet: Data were normalised to reference protein vinculin (1:1000; CST Cat# 13901, RRID: AB_2728768 ), GAPDH (1:500; CST Cat# 2118, RRID: AB_561053 ), or total protein (with the Total Protein detection module) using Compass software (ProteinSimple).
Techniques: Simple Western, Control, Gene Expression, Activation Assay, Isolation
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 
